H11 and 2F3 cell line

Stanford Reference:

13-001

Abstract

Researchers in Dr. Shoshana Levy’s lab have created a pro B cell lines that provides the first B cell lineage tumors in a C57BL mouse strain. The cell lines, known as H11 and 2F3, were made by transducing mouse bone marrow with BCR-ABL retrovirus. The H11 and 2F3 cell lines may be used in immunotherapy and tumor biology research.

Stage of research
The H11 cell line has been used to develop a whole tumor cell vaccine in adoptive cell therapy studies.

Applications

Tumor biology
Immunology

Advantages

First B cell lineage tumors in C57BL strain of mouse
Grows in tissue culture
Transplantable in syngeneic mice
Cell line is MHC class II negative
Cells can be loaded with reagents and labeled for visualization
Can isolate ample numbers of these cells to prepare vaccine

Publications

Goldstein MJ, Varghese B, Brody JD, Rajapaksa R, Kohrt H, Czerwinski DK, Levy S, Levy R. A CpG-loaded tumor cell vaccine induces antitumor CD4+ T cells that are effective in adoptive therapy for large and established tumors. Blood. 2011 Jan 6;117(1):118-27. doi: 10.1182/blood-2010-06-288456. Epub 2010 Sep 27.
Goldstein MJ, Kohrt HE, Houot R, Varghese B, Lin JT, Swanson E, Levy R. Adoptive cell therapy for lymphoma with CD4 T cells depleted of CD137-expressing regulatory T cells. Cancer Res. 2012 Mar 1;72(5):1239-47. doi: 10.1158/0008-5472.CAN-11-3375. Epub 2012 Jan 9.
Building of teh tetraspanin web: distinct structural domains of CD81function in different cellular compartmens. Mol Cell Biol. 2006 Feb; 26(4):1373-85

Innovators & Portfolio

Matthew Goldstein
Shoshana Levy more technologies from Shoshana Levy »

Date Released

12/19/2014 12:00

Licensing Contact

Michael Bellas, Licensing Associate
Request Info

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Related Keywords

B-cell immunology cancer vaccine cancer: lymphoma LS: research tool: cell line LS: research tool: reagent research: cancer tumor immunology

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